Two sympatric phylogroups of the Asian badger Meles leucurus (Carnivora: Mammalia) identified by mitochondrial DNA cytochrome b gene sequences

Koh H. S., Kryukov A., Oh J. G., Bayarkhagva D., Yang B. G., Ahn N. H., Bazarsad D.

P. 1-8

We obtained 20 complete cytochrome b sequences (1140 bp) of Meles leucurus from the Korean Peninsula (mainland Korea and Jeju Island), Russian Far East, and Mongolia, and these sequences were compared to complete and partial (629 bp) sequences of M. leucurus, obtained from GenBank, in order to reexamine genetic differentiation among M. leucurus from Korea, Siberian and Far-eastern Russia, Mongolia, and China. We detected that M. leucurus is composed of two sympatric phylogroups (a major phylogroup from the Urals, Siberia, Mongolia, China, and mainland Korea and a minor phylogroup from China, Far-eastern Russia, mainland Korea, and Jeju Island), indicating that M. leucurus is a monotypic species in the cytochrome b gene. Thus, we concluded that our sequencing results do not support current subspecies classification of M. leucurus, recognizing it as a polytypic species, although further genetic analyses with other markers are needed to confirm our present findings. We also considered that M. leucurus from China and Russian Far East is in contact with M. leucurus from mainland Korea and Jeju Island during the last glacial period, although in both Jeju and Russian Far East the specimens belonging to one of the two phylogroups are found at present. Additionally, we considered that the degree of genetic divergence between insular M. anakuma from Japan and continental M. leucurus from mainland Asia is not so great that our sequencing results do not support current species classification, recognizing M. leucurus as a distinct species: we proposed further systematic analyses to clarify the specific status of M. anakuma.DOI: 10.15298/rusjtheriol.13.1.01

• Abramov A.V. 2002. Variation of the baculum structure of the Palearctic badger (Carnivora, Mustelidae, Meles) // Russian Journal of Theriology. Vol.1. P.57-60.
• Abramov A.V., Puzachenko A.Y. 2006. Geographical variability of skull and taxonomy of Eurasian badgers (Mustelidae, Meles) // Zoologicheskii Zhurnal. Vol.85. P.641-655.
• Allen J.A., Andrew R.C. 1913. Mammals collected in Korea // Bulletin of the American Museum of Natural History. Vol.32. P.427-436.
• Avise J.C., Walker D. 1999. Species realities and numbers in sexual vertebrates: perspectives from an asexual transmitted genome // Proceedings of the National Academy of Sciences of the United States of America. Vol.96. P.992-995.
• Bradley R.D., Baker R.J. 2001. A test of the genetic species concept: cytochrome b sequences and mammals // Journal of Mammalogy. Vol.82. P.960-973.
• Chung C.H. 2007. Vegetation response to climate change on Jeju Island, South Korea, during the last glaciations based on pollen record // Geosciences Journal. Vol.11. P.147-155.
• Goldberg E.E., Land L. 2007. Species and dispersal barriers // American Naturalist. Vol.170. P.297-304.
• Huelsenbeck J.P., Bull J.J., Cunningham C.W. 1996. Combining data in phylogenetic analysis // Trends in Ecology & Evolution. Vol.11. P.152-158.
• Irwin D.M., Kocher T.D., Wilson A.C. 1991. Evolution of the cytochrome b gene of mammals // Journal of Molecular Evolution. Vol.32. P.128-144.
• Johnson K.P., Adler F.R., Cherry J.L. 2000. Genetic and phylogenetic consequences of island biogeography // Evolution. Vol.54. P.387-396.
• Kurose N., Kaneko Y., Abramov A.V., Siriaroonrat B., Masuda R. 2001. Low genetic diversity in Japanese populations of the Eurasian badger Meles meles (Mustelidae, Carnivora) revealed by mitochondrial cytochrome b gene sequences // Zoological Science. Vol.18. P.1145-1151.
• Lomolino M.V., Riddle B.R., Whittaker R.J., Brown J.H. 2010. Biogeography. Fourth edition. Sunderland, MA: Sinauer Associates. 878 p.
• Marmi J., Lopez-Giraldez F., Macdonald D.W., Calafell F., Zholnerovskaya E., Domingo-Roura X. 2006. Mitochondrial DNA reveals a strong phylogeographic structure in the badger across Eurasia // Molecular Ecology. Vol.15. P.1007-1020.
• Mayr E., Ashlock P.D. 1991. Principles of Systematic Zoology. New York: McGraw-Hill Inc. 475 p.
• Park D.W. 1985. [Geology of coasts and mountains in Cheju-Do] // [Cheju-Do Study]. Vol.2. P.321-322 [in Korean].
• Sato J.J., Wolsan M., Prevosti F.J., D'Elia G.D., Begg C., Begg K., Hosoda T., Campbell K.L., Suzuki H. 2012. Evolutionary and biogeographic history of weasel-like carnivores (Musteloidea) // Molecular Phylogenetic and Evolution. Vol.63. P.745-757.
• Sunnucks P. 2000. Efficient genetic markers for population biology // Trends in Ecology & Evolution. Vol.15. P.199-203.
• Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: molecular evolutionary genetic analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods // Molecular Biology and Evolution. Vol.28. P.2731-2739.
• Tashima S., Kaneko Y., Anezaki T., Baba M., Yachimori S., Abramov A.V., Saveljev A.P., Masuda R. 2011. Phylogeographic sympatry and isolation of the Eurasian badgers (Meles, Mustelidae, Carnivora): implications for an alternative analysis using maternally as well paternally inherited genes // Zoological Science. Vol.28. P.293-303.
• Wozencraft W.C. 2005. Order Carnivora // Wilson D.E. & Reeder D.M. (eds.). Mammal Species of the World: a Taxonomic and Geographic Reference. Third edition. Baltimore: Jones Hopkins University Press. P.532-628.
• Zhang Y., Sangke J., Guoqiang J., Shibau L., Zhongyao Y., Genggui W., Manli Z. 1997. Distribution of Mammalian Species in China. Beijing: China Forestry Publishing House. 280 p.