Standard DAPI karyotype of the common shrew Sorex araneus L. (Soricidae, Eulipotyphla)

Minina J.M., Borodin P.M., Searle J.B., Volobouev V.T., Zhdanova N.S.

P. 3-6

A DAPI-banding technique was used to characterise the individual patterns of each chromosome of the Novosibirsk race of the common shrew (Sorex araneus) which has become a model for comparative gene mapping. In order to outline the main identifying characteristics of the individual chromosomes and to make comparisons to previously published G-banded karyotypes, we analysed DAPI-banded chromosomes with different degrees of condensation. On the basis of this analysis we constructed idiograms of chromosomes at a 300 band stage of resolution.DOI: 10.15298/rusjtheriol.6.1.02

• Boyle A.L., Feltquite D.M., Dracopoli N.C., Housman D.E. & Ward D.C. 1992. Rapid physical mapping of cloned DNA on banded mouse chromosomes by fluorescence in situ hybridisation // Genomics. Vol.12. P.106-115.
• Breen M., Bullerdiek J. & Langford C.F. 1999. The DAPI banded karyotype of the domestic dog (Canis familiaris) generated using chromosome-specific paint probes // Chromosome Research. Vol.7. P.401-406.
• Dixkens C., Klett C., Bruch J., Kollak A., Serov O.L., Zhdanova N., Vogel W. & Hameister H. 1998. ZOO-FISH analysis in insectivores: Evolution extols the virtue of the status quo // Cytogenetics and Cell Genetics.Vol.80. P.61-67.
• Halkka L., Halkka O., Skarén U. & Söderlund V. 1974. Chromosome banding pattern in a polymorphic population of Sorex araneus in northeastern Finland // Hereditas. Vol. 76. P.305-314.
• Heng H.H. & Tsui L.C. 1994. FISH detection on DAPI-banded chromosomes // Methods in Molecular Biology. Vol.33. P.35-49.
• ISCN. 1995. An International System for Human Cytogenetic Nomenclature. Mitelman F. (ed.). Basel: S. Karger.
• Král B. & Radjabli S.I. 1974. Banding patterns and Robertsonian fusion in the Western Siberian population of Sorex araneus (Insectivora, Soricidae) // Zoologicke Listy. Vol.25. P.327-334.
• Langford C.F., Fischer P.E., Binns M.M., Holmes N.G. & Carter N.P. 1996. Chromosome-specific paints from a high-resolution flow-karyotype of the dog // Chromosome Research. Vol.4. P.115-123.
• Muller S., Rocchi M., Ferguson-Smith M.A. & Wienberg J. 1997. Toward a multicolor chromosome bar code for the entire human karyotype by fluorescence in situ hybridisation // Humangenetik. Vol.100. P.271-278.
• Olert J. & Schmid M. 1978. Comparative analysis of karyotypes in European shrew species. I. The sibling species Sorex araneus and S. gemellus: Q-bands, G-bands, and position of NORs // Cytogenetics and Cell Genetics. Vol.20. P.308-322.
• Searle J.B., Fedyk S., Fredga K., Hausser J. & Volobouev V.T. 1991. Nomenclature for the chromosomes of the common shrew (Sorex araneus) // Mémoires de la Société Vaudoise des Sciences Naturelles. Vol.19. P.13-22.
• Schweizer D. 1980. Simultaneous fluorescent staining and specific heterochromatic regions (DA-DAPI bands) in human chromosomes // Cytogenetics and Cell Genetics. Vol.27. P.190-193.
• Wόjcik J.M., Ratkiewicz M. & Searle J.B. 2002. Evolution of the common shrew Sorex araneus: chromosome and molecular aspects // Acta Theriologica. Vol.47. Suppl.1. P.139-167.
• Wόjcik J.M., Borodin P.M., Fedyk S., Fredga K, Hausser J., Mishta A., Orlov V.N., Searle J.B., Volobouev V.T. & Zima J. 2003. The list of the chromosome race of the common shrew Sorex araneus (updated 2002) // Mammalia. Vol.67. P.169-178.
• Zhdanova N.S., Karamisheva T.V., Minina J., Astakhova N.M., Lansdorp P., Kammori M., Rubtsov N.B. & Searle J.B. 2005. Unusual distribution pattern of telomeric repeats in the shrews Sorex araneus and Sorex granarius // Chromosome Research. Vol.13. P.617-625.