The distributions of telomeric and ribosomal DNA on the chromosomes of two closely related species, Sorex araneus and Sorex granarius (Soricidae, Eulipotyphla)

The distributions of telomeric and ribosomal DNA on the chromosomes of two closely related species, Sorex araneus and Sorex granarius (Soricidae, Eulipotyphla)

Zhdanova N.S., Minina J.M., Karamysheva T.V., Rubtsov N.B.

P. 007-013

It is widely believed that Robertsonian rearrangements have played a key role in the chromosome evolution of species of the Sorex araneus group. We present FISH data relating to the distribution of telomeric repeats and 18S rDNA on the chromosomes of Sorex araneus and Sorex granarius, which have karyotypes with almost identical chromosome arms. All chromosomes in S. araneus (Novosibirsk race) are metacentrics, whereas S. granarius has an acrocentric karyotype with two metacentric exceptions. In FISH experiments we revealed telomeric repeats at the ends of all S. araneus chromosomes but only on the short arms of S. granarius acrocentrics, which, as we have shown earlier, amount up to 300 kb in length. FISH signals of the (TTAGGG)n probe and the probe derived by microdissection of the pericentric regions of S. granarius acrocentrics a and b were co-localised or sequentially localised on distinct chromatin fibres of S. granarius. 18S rDNA clusters were found at the ends of short arms of 12 out of 16 S. granarius acrocentric pairs. In S. araneus primary cell culture fibroblasts rDNA was found at the ends of the q, t and u arms. However, after long cultivation of these cells an additional FISH signal of rDNA was found at the distal end of the o arm of chromosome go. In some regions the FISH signal of rDNA coincided with the signal of the telomeric probe. We suppose that rapid concerted evolution of telomeric and rDNA led to the repatterning of these repetitive DNA fractions in the sibling species S. araneus and S. granarius as well as the formation of “large” telomeres with unusual structure at the ends of the S. granarius chromosomes.DOI: 10.15298/rusjtheriol.6.1.03

Литература
  • Azzalin C.M., Nergadze S.G. & Giolotto E. 2001. Human intrachromosomal telomeric-like repeats: sequence organization and mechanism of origin // Chromosoma. Vol.110. P.75-82.
  • Castiglia R., Gornung E. & Corti M. 2002. Cytogenetic analyses of chromosomal rearrangements in Mus minutoides/musculoides from North-West Zambia through mapping of the telomeric sequence (TTAGGG)n and banding techniques // Chromosome Research. Vol.10. P.399-406.
  • Dobigny G., Ozouf-Costaz C., Bonillo C. & Volobouev V. 2003. Evolution of rRNA gene clusters and telomeric repeats during explosive genome repatterning in Taterillus X (Rodentia, Gerbillinae) // Cytogenetic and Genome Research. Vol.103. P.94-103.
  • Halkka L. & Söderlund V. 1987. Random NOR-activation in polymorphic and stable chromosomes of Sorex araneus L. // Hereditas. Vol.106. P.293-294.
  • Hartmann N. & Scherthan H. 2004. Characterization of ancestral chromosome fusion points in the Indian muntjac deer // Chromosoma. Vol.112. P.213-220.
  • Garagna S., Zuccotti M., Capanna E. & Redi C.A. 2002. High-resolution organization of mouse telomeric and pericentromeric DNA // Cytogenetic and Genome Research. Vol.96. P.125-129.
  • Ijdo J.W., Wells R.A., Baldini A. & Reeders S.T. 1991. Improved telomere detection using a telomere repeat probe (TTAGGG)n generated by PCR // Nucleic Acids Research. Vol.19. P.4780.
  • Kilburn A.E., Shea M.J., Sargent R.G &, Wilson J.H. 2001. Insertion of a telomere repeat sequence into a mammalian gene causes chromosome instability // Molecular and Cellular Biology. Vol.21. P.126-135.
  • Malygin A.A., Graifer D.M., Zenkova M.A., Mamaev S.V. & Karpova G.G. 1992. [Affinity modification of 80S ribosomes from human placenta by derivatives of triand hexauridylates as mRNA analogs] // Molekulyarnaya Biologiya. Vol.26. P.369-377 [in Russian].
  • Manning E.L., Crosland J., Dewey M.J. & Van Zant G. 2002. Influence of inbreeding and genetics on telomere length in mice // Mammalian Genome. Vol.13. P.234-238.
  • Metcalfe C.J., Eldridge M.D., Toder R. & Johnston P.G. 1998. Mapping of the distribution of the telomeric sequence (TTAGGG)n in the Macropoidea (Marsupialia) by fluorescence in situ hybridization. I. The swamp wallaby, Wallabia bicolor // Chromosome Research. Vol.6. P.603-61.
  • Meyne J., Baker R.J., Hobart H.H., Hsu T.C., Ryder O.A., Ward O.G., Wiley J.E., Wurster-Hill D.H., Yates T.L. & Moyzis R.K.. 1990. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequences in vertebrate chromosomes // Chromosoma. Vol.99. P.3-10.
  • Mondello C., Pirzio L., Azzalin C.M. & Giulotto E. 2000. Instability of interstitial telomeric sequences in the human genome // Genomics. Vol.68. P.111-117.
  • Olert J. & Schmid M. 1978. Comparative analysis of karyotypes in European shrew species. I. The sibling species Sorex araneus and S. gemellus: Q-bands, G-bands, and position of NORs // Cytogenetics and Cell Genetics. Vol.20. P.308-322.
  • Pack S.D., Borodin P.M., Serov O.L. & Searle J.B. 1993. The X-chromosome translocation in the common shrew (Sorex araneus L.): late replication in female somatic cells and pairing in male meiosis // Chromosoma. Vol.102. P.355-360.
  • Rivero M.T., Mosquera A., Goyanes V., Slijepcevic P. & Fernandez J.L. 2004. Differences in repair profiles of interstitial telomeric sites between normal and DNA double-strand break repair deficient Chinese hamster cell // Experimental Cell Research. Vol.295. P.161-172.
  • Rubtsov N., Senger G., Kucera H., Neumann A., Kelbova K., Junker K., Beensen V., Сlaussen U. 1996. Interstitial deletion of chromosome 6q: report of a case and precise definition of the breakpoints by microdissection and reverse painting // Human Genetics. Vol.97. P.705-709.
  • Ruiz-Herrera A., Garcia F., Azzalin C., Giulotto E., Ponsa M. & Garcia M. 2002. Distribution of intrachromosomal telomeric sequences (ITS) on Macaca fascicularis (Primates) chromosomes and their implication for chromosome evolution // Human Genetics. Vol.110. P.578-586.
  • Searle J.B., Fedyk S., Fredga K., Hausser J. & Volobouev V.T. 1991. Nomenclature for the chromosomes of the common shrew (Sorex araneus) // Mémoires de la Société Vaudoise des Sciences Naturelles. Vol.19. P.13-22.
  • Searle J.B. 1993. Chromosomal hybrid zones in eutherian mammals // Harrison R.G. (ed.). Hybrid Zones and the Evolutionary Process. New York: Oxford University Press. P.309-353.
  • Slijepcevic P. 1998. Telomeres and mechanisms of Robertsonian fusion // Chromosoma. Vol.107. P.136-140.
  • Taberlet P., Fumagalli L. & Hausser J. 1994. Chromosomal versus mitochondrial DNA evolution: tracking the evolutionary history of the southwestern European populations of the Sorex araneus group (Mammalia, Insectivora) // Evolution. Vol.48. P.623-636.
  • Volobouev V.T. & Dutrillaux B. 1991. Chromosomal evolution and phylogenetic relationships of the Sorex araneus-arcticus species group // Mémoires de la Société Vaudoise des Sciences Naturelles. Vol.19. P.131-139.
  • Wójcik J.M. & Searle J.B. 1988. The chromosomal complement of Sorex granarius - the ancestral karyotype of the common shrew (Sorex araneus)? // Heredity. Vol.61. P.225-229.
  • Wójcik J.M., Borodin P.M., Fedyk S., Fredga K., Hausser J., Mishta A., Orlov V.N., Searle J.B., Volobouev V.T. & Zima J. 2003. The list of the chromosome races of the common shrew Sorex araneus (updated 2002) // Mammalia. Vol.67. P.169-178.
  • Zhdanova N.S., Karamisheva T.V., Minina Y., Astakhova N.M., Lansdorp P., Kammori M., Rubtsov N.B. & Searle J.B. 2005. Unusual distribution pattern of telomeric repeats in the shrews Sorex araneus and Sorex granarius // Chromosome Research. Vol.13. P.617-625.
  • Zhu L., Hathcock K.S., Hande P., Lansdorp P.M., Seldin M.F. & Hodes R.J. 1998. Telomere length regulation in mice is linked to a novel chromosome locus // Proceedings of the National Academy of Sciences of the USA. Vol.95. P.8648-8653.
  • Zijlmans J.M., Martens U.M., Poon S.S., Raap A.K., Tanke H.J., Ward R.K. & Lansdorp P.M. 1997. Telomeres in the mouse have large inter-chromosomal variations in the number of T2AG3 repeats // Proceedings of the National Academy of Sciences of the USA. Vol.94. P.7423-7428.