Using ISSR markers in determination of genetic relationship between 2n = 54 and 2n = 60 cytotypes of Nannospalax xanthodon (Nordmann, 1840) (Mammalia, Rodentia) from Central Anatolia

Using ISSR markers in determination of genetic relationship between 2n = 54 and 2n = 60 cytotypes of Nannospalax xanthodon (Nordmann, 1840) (Mammalia, Rodentia) from Central Anatolia

Yağcı T., Şen E., Gurbanov R.R.

P. 100-107

A total of 36 samples of 2n = 54 and 2n = 60 cytotypes of Nannospalax xanthodon, distributed in the Central Anatolia region of Turkey, were analyzed for the first time by using inter-simple sequence repeat-polymerase chain reaction (ISSR-PCR) technique. The analysis revealed 112 ISSR bands, 101 of which were polymorphic. Seven ISSR primers ((AG)8 T, (GGAGA)5, (GACA)4, (TG)8 A, (CAG)5 GC, (CAG)4 AC and (GA)8 AC)) were optimized from total 20 primers. (AG)8 T and (GA)8 AC primers were most informative to distinguish cytotypes by producing specific bands for 2n = 54 and 2n = 60. The cytotypes in genetically close relationships were separated into three different groups by UPGMA cluster analysis, in which, the highest genetic diversity was measured for 2n = 60. Our results showed that ISSR markers can be used as a simple and reliable molecular tool, for the estimation of genetic diversity in cytotypes of Nannospalax at low range genetic distances.DOI: 10.15298/rusjtheriol.17.2.06

Literature
  • Arslan A. & Bölükbaş F. 2010. C-heterochromatin and NORs distribution of mole rat, Nannospalax xanthodon from Aksaray, Turkey // Caryologia. Vol.63. P.398–404.
  • Arslan E., Gülbahçe E., Arıkoğlu H., Arslan A., Bužan E.V. & Kryštufek B. 2010. Mitochondrial divergence between three cytotypes of the Anatolian mole rat, Nannospalax xanthodon // Zoology in the Middle East. Vol.50. P.27–34.
  • Arslan A. & Zima J. 2014. Karyotypes of the mammals of Turkey and neighbouring regions: a review // Folia Zoologica. Vol.63. P.1–62.
  • Buczkowska K., Rabska M., Gonera P., Pawlaczyk E.M., Wawrzyniak P., Czołpińska M. & Bączkiewicz A. 2016. Effectiveness of ISSR markers for determination of the Aneura pinguis cryptic species and Aneura maxima // Biochemical Systematics and Ecology. Vol.68. P.27–35.
  • Bugarski-Stanojević V., Blagojević J., Adnağević T., Jojić V. & Vujośević M. 2008. Molecular phylogeny and distribution of three Apodemus species (Muridae, Rodentia) in Serbia // Journal of Zoological Systematics and Evolutionary Research. Vol.46. P.278–286.
  • Bugarski-Stanojević V., Blagojević J., Stamenković G., Adnađević T., Giagia-Athanasopoulou E.B. & Vujošević M. 2011. Comparative study of the phylogenetic structure in six Apodemus species (Mammalia, Rodentia) inferred from ISSR-PCR data // Systematics and Biodiversity. Vol.9. P.95–106.
  • Gromov I.M. & Baranova G.I. (eds.). 1981. [Catalogue of Mammals of USSR (Pliocene to Recent)]. Leningrad: Nauka. 456 p. [In Russian]
  • Hadid Y., Nemeth A., Snir S., Pavlicek T., Csorba G., Kazmer M., Major A., Mezhzherin S., Rusin M. & Coşkun Y. 2012. Is evolution of blind mole rats determined by climate oscillations? // PLoS ONE. Vol.7. P.1–7.
  • Ivanitskaya E., Sцzen M., Rashkovetsky L., Matur F. & Nevo E. 2008. Discrimination of 2n=60 Spalax leucodon cytotypes (Spalacidae, Rodentia) in Turkey by means of classical and molecular cytogenetic techniques // Cytogenetic and Genome Research. Vol.122. P.139–149.
  • Jojić V., Bugarski-Stanojević V., Blagojević J., & Vujošević M. 2014. Discrimination of the sibling species Apodemus flavicollis and A. sylvaticus (Rodentia, Muridae) // Journal of Comparative Zoology. Vol.253. P.261–269.
  • Joshi S.P., Gupta V.S., Aggarwal R.K., Ranjekar P.K. & Brar D.S. 2000. Genetic diversity and phylogenetic relationship as revealed by inter simple sequence repeat (ISSR) polymorphism in the genus Oryza // Theoretical and Applied Genetics. Vol.100. P.1311–1320.
  • Kandemir Э., Sözen M., Matur F., Kankılıç T., Martнnkova N., Зolak R., Цzkurt S.Ц. & Зolak E. 2012. Phylogeny of species and cytotypes of mole rats (Spalacidae) in Turkey inferred from mitochondrial cytochrome b gene sequences // Folia Zoologica. Vol.61. P.25–33.
  • Kankılıç Te. & Gürpınar C. 2014. Revised classification design of the Anatolian species of Nannospalax (Rodentia: Spalacidae) using RFLP analysis // Turkish Journal of Zoology. Vol.38. P.68–78.
  • Kankılıç Te., Kankılıç To., Sözen M. & Çolak E. 2013. Genetic diversities and geographic variations of chromosomal forms of Nannospalax nehringi (Satunin, 1898) and Nannospalax ehrenbergi (Nehring, 1898) from Turkey, revealed by RAPD analysis // Acta Zoologica Bulgarica. Vol.65. P.45–58.
  • Kankılıç Te., Kankılıç To., Sözen M. & Çolak E. 2015. Allozyme variations in Anatolian populations and cytotypes of the blind mole rats (Nannospalax) // Biochemical Systematics and Ecology. Vol.59. P.126–134.
  • Kankılıç Te., Arslan Atilla, Şeker P.S., Kankılıç To., Toyran K. & Zima J. 2017. A new chromosomal race (2n =44) of Nannospalax xanthodon from Turkey (Mammalia: Rodentia) // Zoology in the Middle East. Vol.63. No.3. P.181–188.
  • Klein Hofmeijer G. & de Bruijn H. 1985. The mammals from the Lower Miocene of Aliveri (Island of Evia, Greece). IV: The Spalacidae and Anomalomyidae // Proceedings of the Koninklijke Nederlandse Akademie van Wetenschappen. Series B. Palaeontology, Geology, Physics, Chemistry, Anthropology. Vol.88. No.2. P.185–198.
  • Kryštufek B. & Vohralik V. 2009. Cricetinae, Muridae, Spalacidae, Calomyscidae, Capromyidae, Hystricidae, Castoridae // Mammals of Turkey and Cyprus. Rodentia II. Koper, Slovenia: Knjiћnica Annales Majora. P.242–280.
  • Kryštufek B., Ivanitskaya E., Arslan A., Arslan E. & Buzan E. 2012. Evolutionary history of mole rats (genus Nannospalax) inferred from mitochondrial cytochrome b sequence // Biological Journal of the Linnean Society. Vol.105. No.2. P.446–455.
  • Matur F., Çolak F., Sevindik M. & Sözen M. 2011. Chromosome differentiation of four 2n=50 chromosomal forms of Turkish mole rat, Nannospalax nehringi // Zoological Science. Vol.28. P.61–67.
  • Nei M. 1978. Estimation of average heterozygosity and genetic distance from a small number of individuals // Genetics. Vol.89. P.583–590.
  • Nevo E. 1991. Evolutionary theory and processes of active speciation and adaptive radiation in subterranean mole rats, Spalax ehrenbergi superspecies, in Israel // Evolutionary Biology. Vol.2. P.1-125.
  • Nevo E., Filippucci M.G., Redi C., Korol A. & Beiles A. 1994. Chromosomal speciation and adaptive radiation of mole rats in Asia Minor correlated with increased ecological stress // Proceedings of the National Academy of Sciences of the USA. Vol.91. P.8160–8164.
  • Nevo E., Filippucci M.G., Redi C., Simson S., Heth G. & Beiles A. 1995. Karyotype and genetic evolution in speciation of subterranean mole rats of the genus Spalax in Turkey // Biological Journal of the Linnean Society. Vol.54. P.203–229.
  • Savic I. & Nevo E. 1990. The Spalacidae: Evolutionary history, speciation, and population biology // Nevo E. & Reig O.A. (eds.). Evolution of Subterranean Mammals at the Organismal and Molecular Levels. New York: Alan R. Liss, Inc. P.129–143.
  • Sözen M., Çataklý K., Eroğlu F., Matur F. & Sevindik M. 2011. Distribution of chromosomal forms of Nannospalax nehringi (Satunin, 1898) (Rodentia: Spalacidae) in Çankýrý and Çorum provinces, Turkey // Turkish Journal of Zoology. Vol.35. No.3. P.367–374.
  • Sözen M., Çolak F., Sevindik M. & Matur F. 2013. Cytotypes of Nannospalax xanthodon (Satunin, 1898) (Rodentia: Spalacidae) from western Anatolia // Turkish Journal of Zoology. Vol.37. P.462–469.
  • Sözen M., Çolak F., Sevindik M. & Matur F. 2015. Two new cytotypes and some additional karyologэcal records for Anatolian mole rats of the Nannospalax xanthodon and Nannospalax ehrenbergi (Mammalia, Rodentia) in Turkey // Folia Zoologica. Vol.64. No.2. P.167–172.
  • Styan C.A., McCluskey C.F., Sun Y. & Kupriyanova E.K. 2017. Cryptic sympatric species across the Australian range of the global estuarine invader Ficopomatus enigmaticus (Fauvel, 1923) (Serpulidae, Annelida) // Aquatic Invasions. Vol.12. No.1. P.53–65.
  • Suzuki H., Wakana S., Yonekawa H., Moriwaki K., Sakurai S. & Nevo E. 1996. Variations in ribosomal DNA and mitochondrial DNA among chromosomal species of subterranean mole rats // Molecular Biology and Evolution. Vol.13. P.85–92.
  • Topachevskii V.A. 1969. [Mole rats Spalacidae] // Fauna SSSR. Mlekopitayushchie. Vol.3. No.3. Leningrad: Nauka. 308 p. [In Russian]
  • Wahrman J., Goitein R. & Nevo E. 1969. Geographic variation of chromosome forms in Spalax, a subterranean rodent of restricted mobility // Benirschke K. (ed.). Comparative Mammalian Cytogenetics. Berlin, Heidelberg: Springer. P.30–48.
  • Yağcı T. 2010. [G-banding and allozyme variations of Nannospalax leucodon (Nordmann, 1840) in Central Anatolia Region (Mammalia: Rodentia)] // PhD Thesis. Kýrýkkale University. Kýrýkkale. 103 p. [in Turkish].
  • Yeh F.C., Yang R.C. & Boyle T. 1999. Popgene Version 1.31 Microsoft Windows-based Software for Population Genetics Analysis. Alberta, Canada: University of Alberta and Centre for International Forestry Research. 28 p.
  • Yiğit N., Çolak E. & Sözen M. 1999. Habitats of Turkish rodents (Mammalia: Rodentia) and their effects of cultivated fields // Journal of the Institute of Science Technology, Gazi Üni. Vol.12. No.4. P.885–903.
  • Yiğit N., Çolak E., Sözen M. & Karataş A. 2006. Rodents of Türkiye. Ankara: Meteksan Co. 154 p.
  • Yüksel E. & Gülkaç M.D. 1990. The evolution and phylogenetic relationship in some subspecies and chromosomal forms of Spalax leucodon // Turkish Journal of Biology. Vol.14. P.59–68.
  • Zhigileva O.N. & Gorbacheva E.V. 2017. Distribution and parameters of genetic polymorphism in northern red-backed vole (Clethrionomys rutilus) and bank vole (Clethrionomys glareolus) in West Siberia // Contemporary Problems of Ecology. Vol.10. P.1–8.
  • Zhigileva O.N., Politov D.V., Golovacheva I.M. & Petrovicheva S.V. 2014. Genetic variability of sable Martes zibellina L., pine marten M. martes L. and their hybrids in West Siberia: polymorphism of proteins and DNA // Russian Journal of Genetics. Vol.50. P.508–517.
  • Zietkiewicz E., Rafalski A. & Labuda D. 1994. Genome fingerprinting by simple sequence repeats (SSR)-anchored PCR amplification // Genomics. Vol.20. P.176–183.